BIOT 2420 - Biotechnology Techniques II

4 sem hrs cr

(2 hours lecture, 3 hours lab)

The second semester of a two-semester project-oriented course applying the fundamental DNA and protein manipulation techniques used in biotechnology/molecular biology research-oriented laboratories in academia and industry. This course concentrates on DNA structure and function and the techniques of DNA analysis, including cloning, restriction digests, and polymerase chain reactions.  Prerequisite: BIOT 2410  with a grade of “C” or better.

In rare and unusual circumstances, a course prerequisite can be overridden with the permission of the Department Lead for the discipline.

Transfer (UT) or Non-Transfer Course (UN): UN

Upon successful completion of this course, students will be able to…

• demonstrate an understanding of protein structure and function. 
• operate basic laboratory equipment needed for protein analysis
• show proficiency in SDS protein electrophoresis.
• demonstrate an understanding of the process of Western Blotting. 
• demonstrate basic mathematical skills used for preparation of solutions in the lab.